Journal: Oxidative Medicine and Cellular Longevity

Article Title: Intragastric Application of Aspirin, Clopidogrel, Cilostazol, and BPC 157 in Rats: Platelet Aggregation and Blood Clot

doi: 10.1155/2019/9084643

Figure Lengend Snippet: Rats which underwent antithrombotic agent aspirin (10 mg/kg intragastrically, once daily for three days) received immediately thereafter BPC 157 (10 μ g/kg intragastrically, once daily for three days) (white bars) or an equal volume of saline (5 ml/kg, intragastrically, once daily for three days) (gray bars); they were sacrificed at 2 h after the last application. Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany). Typical parameters obtained are clotting time (CT), the time from the beginning of measurement until the clot starts to form; clot formation time (CFT), the time needed for the clot to reach an amplitude of 20 mm; alpha-angle, angle of tangent at 2 mm amplitude; maximum clot firmness (MCF), the maximum amplitude of the curve during 60 minutes of measurement; Ly30, clot lysis at 30 minutes; and maximum lysis (ML) which describes the percentage of the maximum lost clot firmness relative to MCF. We analyzed the external pathway with tissue factor (EXTEM), an intrinsic pathway with ellagic acid (INTEM), or without platelet contribution with cytochalasin D (FIBTEM). After 60 minutes, CT, CFT, alpha-angle, MCF, Ly30, and ML were recorded. P > 0.05, vs. control.

Article Snippet: Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany).

Techniques: Modification, Coagulation, Lysis, FIBTEM Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Intragastric Application of Aspirin, Clopidogrel, Cilostazol, and BPC 157 in Rats: Platelet Aggregation and Blood Clot

doi: 10.1155/2019/9084643

Figure Lengend Snippet: Rats which underwent antithrombotic agent cilostazol (10 mg/kg intragastrically, once daily for three days) received immediately thereafter BPC 157 (10 μ g/kg intragastrically, once daily for three days) (white bars) or an equal volume of saline (5 ml/kg, intragastrically, once daily for three days) (gray bars); they were sacrificed at 2 h after the last application. Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany). Typical parameters obtained are clotting time (CT), the time from the beginning of measurement until the clot starts to form; clot formation time (CFT), the time needed for the clot to reach an amplitude of 20 mm; alpha-angle, angle of tangent at 2 mm amplitude; maximum clot firmness (MCF), the maximum amplitude of the curve during 60 minutes of measurement; Ly30, clot lysis at 30 minutes; and maximum lysis (ML) which describes the percentage of the maximum lost clot firmness relative to MCF. We analyzed external pathway with tissue factor (EXTEM), an intrinsic pathway with ellagic acid (INTEM), or without platelet contribution with cytochalasin D (FIBTEM). After 60 minutes CT, CFT, alpha-angle, MCF, Ly30, and ML were recorded. P > 0.05, vs. control.

Article Snippet: Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany).

Techniques: Modification, Coagulation, Lysis, FIBTEM Assay

Journal: The journal of trauma and acute care surgery

Article Title: Effects of platelet-sparing leukocyte reduction and agitation methods on in vitro measures of hemostatic function in cold-stored whole blood

doi: 10.1097/TA.0000000000001870

Figure Lengend Snippet: Viscoelastic measures in nonleukoreduced and leukoreduced WB units over time. This figure shows viscoelastic measures of non-LR (blue bars) and LR (green bars) whole blood units over 15 days. These include the MCF measured by ROTEM (A), MA measured by TEG (B), TEG MA-ADP (C), and TEG MA-AA (D). Median measurements of MCF, TEG MA, TEG MA-ADP, and TEG MA-AA were compared at each time point between LR and non-LR groups, and absolute median measurements were compared from Day 0 to Day 15 within LR or non-LR groups. Differences are indicated by *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Rotational Thromboelastometry (ROTEM) Clot formation was analyzed using a ROTEM delta WB analyzer (Munich, Germany) following the manufacturer’s instructions.

Techniques:

Journal: The journal of trauma and acute care surgery

Article Title: Effects of platelet-sparing leukocyte reduction and agitation methods on in vitro measures of hemostatic function in cold-stored whole blood

doi: 10.1097/TA.0000000000001870

Figure Lengend Snippet: Viscoelastic measures in leukoreduced WB units by agitation method over time. This figure shows viscoelastic measures of LR WB units by differing agitation method over 15 days. Agitation methods included un-rocked (blue bar; none), up and down shake (green bar), continuous mechanical horizontal agitation via flat rocker (yellow bar), or continuous end-over-end rocking via a 360° rotator (red). The viscoelastic parameters measured include the MCF measured by ROTEM (A), MA measured by TEG, (B), TEG MA-ADP (C), and TEG MA-AA (D). Median measurements of MCF, TEG-MA, TEG MA-ADP, and TEG MA-AA were compared at each time point. No differences were noted. Median measurements of MCF, TEG-MA, TEG MA-ADP, and TEG MA-AA were compared within each agitation group from Day 0 to Day 15. Differences are indicated by *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Rotational Thromboelastometry (ROTEM) Clot formation was analyzed using a ROTEM delta WB analyzer (Munich, Germany) following the manufacturer’s instructions.

Techniques:

Journal: The journal of trauma and acute care surgery

Article Title: Effects of platelet-sparing leukocyte reduction and agitation methods on in vitro measures of hemostatic function in cold-stored whole blood

doi: 10.1097/TA.0000000000001870

Figure Lengend Snippet: Viscoelastic measures in nonleukoreduced WB units by agitation method over time. This figure shows viscoelastic measures of non-LR WB units by differing agitation method over 15 days. Agitation methods included un-rocked (blue bar; none), up and down shake (green bar), continuous mechanical horizontal agitation via flat rocker (yellow bar), or continuous end-over-end rocking via a 360° rotator (red). Viscoelastic parameters measured include MCF measured by ROTEM (A), MA measured by TEG (B), TEG MA-ADP (C), and TEG MA-AA (D). Median measurements of MCF, TEG-MA, TEG MA-ADP, and TEG MA-AA were compared at each time point, and absolute median measurements were compared within each agitation method form Day 0 to Day 15.Differences are indicated by *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Rotational Thromboelastometry (ROTEM) Clot formation was analyzed using a ROTEM delta WB analyzer (Munich, Germany) following the manufacturer’s instructions.

Techniques: